Which statement is true about the euthanasia of neonatal mice?

The assertion that an anesthetic overdose is an acceptable method of euthanasia for neonatal mice is accurate because euthanasia must be performed in a manner that minimizes suffering. Anesthetic overdose achieves this by ensuring the animal remains unconscious and does not experience distress during the process. Using an overdose of anesthetics is a recognized method, provided it is administered in a manner consistent with guidelines provided by organizations that oversee animal care and use, such as the American Veterinary Medical Association (AVMA).

In the context of neonatal mice, euthanasia protocols often need to cater to their developmental stage and physiological characteristics. Therefore, methods that are humane and effective, like anesthetic overdose, are vital for ethical animal research practices.

The other statements do not align with established practices. While it is important for all euthanasia procedures to have received IACUC approval, which aligns with institutional regulations regarding animal care and welfare, this does not encapsulate the sole criteria for euthanasia methods. Lastly, while all euthanasia methods must indeed be humane and comply with applicable guidelines, the statement regarding anesthetic overdose directly addresses its acceptability and practical application, reinforcing why it is a valid choice.