Which statement about blood collection in mice and rats is TRUE?

The statement indicating that lateral saphenous, facial, and jugular vein collection are less painful alternatives stands true because these methods are generally considered to be more humane options for blood collection in mice and rats.

When assessing pain and discomfort associated with blood collection techniques, it is crucial to recognize that some methods, such as retroorbital bleeding, can cause greater distress and discomfort to the animal, making them less desirable. Lateral saphenous and facial vein collections, as well as jugular vein methods, can often be performed with less invasive approaches that minimize the potential for pain to the animal, given that they are typically conducted in a manner that provides better control and minimizes stress.

Additionally, these methods are advocated in various animal care guidelines, highlighting a preference for techniques that prioritize animal welfare while still allowing researchers to obtain necessary biological samples. It is important to adhere to established protocols and guidelines to ensure the ethical treatment of animals in research contexts, emphasizing the importance of selecting the least distressing methods available.