What is required of the principal investigator by the IACUC regarding alternatives?

The requirement for the principal investigator to ensure that alternatives to painful procedures have been considered is a fundamental aspect of the ethical use of animals in research. This mandate stems from the principles of the 3Rs: Replacement, Reduction, and Refinement.

The IACUC is responsible for reviewing research proposals to ensure that researchers have explored all possible alternatives to using animals or have minimized the number of animals used and reduced their potential suffering. This involves evaluating whether there are non-animal methods available that could achieve similar research goals or whether procedures can be modified to lessen pain and distress. By focusing on alternatives, the IACUC supports the ethical obligation to treat animals with respect and care, conforming to established guidelines and regulations that govern animal research.

The other options do not address the specific ethical considerations related to alternatives in research practices.