Adjunctive methods of euthanasia, such as pithing frogs, should only be performed on animals that are already unconscious.

The assertion that adjunctive methods of euthanasia, such as pithing frogs, should only be performed on animals that are already unconscious is based on ethical considerations and animal welfare principles. This practice ensures that the animal does not experience undue suffering during the process of euthanasia.

Performing such procedures on conscious animals would conflict with humane treatment standards that prioritize minimizing pain and distress. Therefore, conducting pithing on an unconscious animal aligns with the objective of ensuring that the euthanasia process is as humane as possible. This is why the training and protocols established by bodies like the IACUC emphasize the importance of confirming an animal's unconsciousness prior to performing adjunctive euthanasia methods.